Expression of New Recombinant IgG-Binding Polypeptides and Analysis of Their Capacity to Bind Human IgG
- Autores: Bormotova E.1, Gupalova T.1
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Afiliações:
- Department of Molecular Microbiology, Institute of Experimental Medicine
- Edição: Volume 165, Nº 3 (2018)
- Páginas: 373-377
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240316
- DOI: https://doi.org/10.1007/s10517-018-4173-z
- ID: 240316
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Resumo
Protein G is present in group G streptococcus strain (G4223); the IgG-binding part of this protein contains three IgG-binding domains and binds human IgG with very high activity. We obtained two recombinant polypeptides G4223 and G14223 with high IgG-binding activity. Polypeptide G14223 consisting of three IgG-binding domains and W region has higher molecular weight and is characterized by higher affinity for IgG than polypeptide G4223 consisting of only three IgG-binding domains. It was shown that polypeptide affinity depends on its structure and size.
Sobre autores
E. Bormotova
Department of Molecular Microbiology, Institute of Experimental Medicine
Email: tvgupalova@rambler.ru
Rússia, St. Petersburg
T. Gupalova
Department of Molecular Microbiology, Institute of Experimental Medicine
Autor responsável pela correspondência
Email: tvgupalova@rambler.ru
Rússia, St. Petersburg