Study of the Efficiency of the Hydroporation for Delivery of Plasmid DNA to the Cells on the Model of Toxic Neuropathy
- Autores: Yudin M.1, Bykov V.1, Nikiforov A.1, Al-Shekhadat R.2, Ivanov I.1, Ustinova T.1
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Afiliações:
- State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
- InnovaBio Company
- Edição: Volume 164, Nº 6 (2018)
- Páginas: 798-802
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239922
- DOI: https://doi.org/10.1007/s10517-018-4083-0
- ID: 239922
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Resumo
We compared the efficiency of delivery of plasmid DNA (active ingredient concentration 1 mg/kg) that provides production of nerve growth factor (NGF) after intravenous administration to rats and after administration by hydroporation. The method of hydroporation ensured plasmid penetration into the liver tissue and lengthened the time of its detection in the organ. DNA concentration in 1 h after its introduction by hydroporation or intravenous route was 0.7 and 0.05 ng/mg tissue, respectively. The use of this transfection method ensured preservation of NGF DNA in the liver tissue at a level of 0.24 ng/mg of tissue 1 day after administration of the plasmid construct, while after intravenous administration, expression of the analyzed DNA was not detected in blood and liver samples. After hydroporation, the maximum of relative normalized expression of cDNA (270 rel. units) was observed after 4 h, and after 1 day, this parameter decreased to 35 rel. units. Introduction of plasmid DNA of NGF by hydroporation prevented the development of disorders of neuromuscular conduction in a rats model of toxic neuropathy induced by subacute administration of malathion in a dose of 0.5 LD50.
Sobre autores
M. Yudin
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Autor responsável pela correspondência
Email: gniii_vm2@mil.ru
Rússia, St. Petersburg
V. Bykov
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Email: gniii_vm2@mil.ru
Rússia, St. Petersburg
A. Nikiforov
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Email: gniii_vm2@mil.ru
Rússia, St. Petersburg
R. Al-Shekhadat
InnovaBio Company
Email: gniii_vm2@mil.ru
Rússia, St. Petersburg
I. Ivanov
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Email: gniii_vm2@mil.ru
Rússia, St. Petersburg
T. Ustinova
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Email: gniii_vm2@mil.ru
Rússia, St. Petersburg
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