Effect of Pharmacological Modulation of Activity of Metabotropic Glutamate Receptors on Their Gene Expression after Excitotoxic Damage in Hippocampal Neurons
- Autores: Pershina E.1,2, Kapralova M.1,2, Arkhipov V.1,2
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Afiliações:
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
- Pushchino State Natural Science Institute
- Edição: Volume 162, Nº 6 (2017)
- Páginas: 784-787
- Seção: Genetics
- URL: https://journals.rcsi.science/0007-4888/article/view/238565
- DOI: https://doi.org/10.1007/s10517-017-3713-2
- ID: 238565
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Resumo
Microinjection of kainic acid into rat hippocampus causes excitotoxic neuronal damage predominantly in the CA3 and CA1 fields. These lesions can be significantly reduced by simultaneous administration of MPEP, a negative allosteric modulator of type 5 metabotropic glutamate receptors, and LY354740, an agonist of type 2 metabotropic glutamate receptors. The decrease in neuronal death in the hippocampus during pharmacological modulation was paralleled by adaptive changes in gene expression. In the hippocampus, gene expression of type 5 postsynaptic metabotropic glutamate receptor was close to the control level, and in the frontal cortex expression of the gene of α1-subunit of the GABAA receptor returned to normal. In the frontal cortex, a reciprocal relationship was observed for type 2 metabotropic glutamate receptor: expression of the corresponding gene decreased in response to pharmacological activation.
Sobre autores
E. Pershina
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Pushchino State Natural Science Institute
Email: viarkhipov@rambler.ru
Rússia, Pushchino
M. Kapralova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Pushchino State Natural Science Institute
Email: viarkhipov@rambler.ru
Rússia, Pushchino
V. Arkhipov
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Pushchino State Natural Science Institute
Autor responsável pela correspondência
Email: viarkhipov@rambler.ru
Rússia, Pushchino