Effects of Release-Active Antibodies to CD4 Receptor on the Level of lck-Kinase in Cultured Mononuclear Cells from Human Peripheral Blood
- Autores: Emel’yanova A.1, Grechenko V.2, Petrova N.1, Shilovskii I.3, Gorbunov E.2, Tarasov S.2, Khaitov M.3, Morozov S.1, Epshtein O.1
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Afiliações:
- 1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology
- Research and Production Company Materia Medica Holding
- State Research Centre Institute of Immunology, Federal Medical-Biological Agency of the Russian Federation
- Edição: Volume 162, Nº 3 (2017)
- Páginas: 323-326
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238168
- DOI: https://doi.org/10.1007/s10517-017-3606-4
- ID: 238168
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Resumo
For evaluation of effects of release-active antibodies to CD4 on cultured lymphocytes from human peripheral blood, we measured intracellular content of lck-kinase cell-based ELISA. In cells treated with release-active antibodies to CD4, the content of intracellular lck-kinase significantly (p<0.01) decreased in comparison with the control (purified water processed in a similar way). Phytohemagglutinin had no effect on the concentration of lck-kinase in cells. The decrease in the content of CD4-associated lck protein suggests that the preparation enhanced intracellular coupling of lck-kinase with T-cell receptor and potentiated T-cell immune response.
Sobre autores
A. Emel’yanova
1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology
Autor responsável pela correspondência
Email: agemelyanova@gmail.com
Rússia, Moscow
V. Grechenko
Research and Production Company Materia Medica Holding
Email: agemelyanova@gmail.com
Rússia, Moscow
N. Petrova
1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology
Email: agemelyanova@gmail.com
Rússia, Moscow
I. Shilovskii
State Research Centre Institute of Immunology, Federal Medical-Biological Agency of the Russian Federation
Email: agemelyanova@gmail.com
Rússia, Moscow
E. Gorbunov
Research and Production Company Materia Medica Holding
Email: agemelyanova@gmail.com
Rússia, Moscow
S. Tarasov
Research and Production Company Materia Medica Holding
Email: agemelyanova@gmail.com
Rússia, Moscow
M. Khaitov
State Research Centre Institute of Immunology, Federal Medical-Biological Agency of the Russian Federation
Email: agemelyanova@gmail.com
Rússia, Moscow
S. Morozov
1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology
Email: agemelyanova@gmail.com
Rússia, Moscow
O. Epshtein
1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology
Email: agemelyanova@gmail.com
Rússia, Moscow