Mesenchymal-Epithelial Transition in Culture of Stromal Progenitor Cells Isolated from the Liver of a Patient with Alcoholic Cirrhosis
- Autores: Kholodenko I.1, Kholodenko R.2, Manukyan G.3, Burunova V.1, Yarygin K.1
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Afiliações:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- B. V. Petrovsky Russian National Research Center of Surgery, Russian Academy of Medical Sciences
- Edição: Volume 162, Nº 1 (2016)
- Páginas: 115-119
- Seção: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/237969
- DOI: https://doi.org/10.1007/s10517-016-3559-z
- ID: 237969
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Resumo
The cells isolated from biopsy specimen of a patient with alcoholic liver cirrhosis and cultured under standard conditions for obtaining stromal cell culture clearly diverged during early passages into two morphologically and phenotypically different subtypes: epithelial and mesenchymal. Mesenchymal cells expressed CD90 and CD44 and epithelial cells expressed CD166, CD227, and hepatocyte growth factor receptor Met. Starting from passage 6, the culture underwent spontaneous morphological changes and by passages 8-10 contained only epithelium-like cells. CD90 and CD44 expression disappeared, CD166 and CD227 expression remained unchanged, and Met expression increased. A small fraction of cells expressed GATA-4, HNF3β, HNF1α, and HNF4α. After addition of inducers of hepatogeneic differentiation, the cells started producing albumin.
Sobre autores
I. Kholodenko
V. N. Orekhovich Research Institute of Biomedical Chemistry
Autor responsável pela correspondência
Email: irkhol@yandex.ru
Rússia, Moscow
R. Kholodenko
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: irkhol@yandex.ru
Rússia, Moscow
G. Manukyan
B. V. Petrovsky Russian National Research Center of Surgery, Russian Academy of Medical Sciences
Email: irkhol@yandex.ru
Rússia, Moscow
V. Burunova
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: irkhol@yandex.ru
Rússia, Moscow
K. Yarygin
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: irkhol@yandex.ru
Rússia, Moscow
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