Expression of Apoptosis Regulator Proteins Bcl-2 and Bad in Rat Ovarian Follicular Apparatus during Recovery after Extreme Hypothermia
- Авторлар: Michurina S.1, Kolesnikov S.2,3, Bochkareva A.1, Ishchenko I.1, Arkhipov S.1
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Мекемелер:
- Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
- Research Center of Family Health and Human Reproduction Problems
- M. V. Lomonosov Moscow State University
- Шығарылым: Том 168, № 2 (2019)
- Беттер: 205-209
- Бөлім: General Pathology and Pathophysiology
- URL: https://journals.rcsi.science/0007-4888/article/view/242364
- DOI: https://doi.org/10.1007/s10517-019-04675-x
- ID: 242364
Дәйексөз келтіру
Аннотация
The expression of molecular and cellular regulators of apoptosis (proapoptotic protein Bad and antiapoptotic protein Bcl-2) was measured in the follicular apparatus of rat ovaries during the recovery period (days 7 and 14) after hyperthermia (up to rectal temperature 43.5°C). The Bcl-2/Bad index was calculated. The expression of Bcl-2 in the follicular apparatus of rat ovaries increased on day 7 after the exposure. The Bcl-2/Bad index also increased, which suggests that the development of apoptosis by the mitochondrial pathway in follicles was limited at this term after hyperthermia. On day 14 after hyperthermia, the area of immunohistochemical staining for the antiapoptotic protein Bcl-2 significantly decreased in cells of the ovarian follicular epithelium, but the expression of the proapoptotic protein Bad significantly increased; these changes led to a decrease in Bcl-2/Bad index, which attested to weakening of the antiapoptotic defense and activation of oocyte apoptosis by the mitochondrial pathway.
Авторлар туралы
S. Michurina
Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: s.michurina@ngs.ru
Ресей, Novosibirsk
S. Kolesnikov
Research Center of Family Health and Human Reproduction Problems; M. V. Lomonosov Moscow State University
Email: s.michurina@ngs.ru
Ресей, Irkutsk; Moscow
A. Bochkareva
Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: s.michurina@ngs.ru
Ресей, Novosibirsk
I. Ishchenko
Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: s.michurina@ngs.ru
Ресей, Novosibirsk
S. Arkhipov
Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: s.michurina@ngs.ru
Ресей, Novosibirsk