In Vitro Analysis of the Expression of CD11, CD29, CD36, and DC-STAMP Molecules during the Formation of Multinuclear Macrophages in BCG-Infected Mice


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Expression of CD11, CD29, CD36, and DC-STAMP molecules by macrophages was analyzed in in vitro experiments. These molecules mediate cell fusion, one of the mechanisms underlying the formation of multinuclear macrophages. Macrophages were obtained from intact and BCG-infected male BALB/c mice. In intact cultures, multinuclear macrophages appeared primarily due to amitotic division of cell nuclei, while in macrophage cultures from infected mice, the process of cell fusion predominated. In intact macrophage cultures, bi- and multinuclear cells expressed primarily CD29 and CD36. In cultures from infected mice, macrophages expressing CD29 and DC-STAMP predominated, but bi- and multinuclear macrophages expressing CD11 and CD36 predominated over mononuclear ones. The study of macrophage fusion mechanism can be useful for understanding of this biological phenomenon as the mechanisms of delivery of M. tuberculosis and lysosomotropic anti-tuberculosis drugs into tuberculous granulomas to suppress M. tuberculosis persisting in macrophages and reduce the destructive potential of granulomas.

Авторлар туралы

D. Il’in

Research Institute of Experimental and Clinical Medicine, Federal Research Center of Fundamental and Translational Medicine

Хат алмасуға жауапты Автор.
Email: ilindenis.ilin@yandex.ru
Ресей, Novosibirsk

V. Shkurupy

Research Institute of Experimental and Clinical Medicine, Federal Research Center of Fundamental and Translational Medicine; Novosibirsk State Medical University, Ministry of Health of the Russian Federation

Email: ilindenis.ilin@yandex.ru
Ресей, Novosibirsk; Novosibirsk


© Springer Science+Business Media, LLC, part of Springer Nature, 2019

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