Analysis of the Action of Quaternized Chitosans with Different Molecular Weight on Anticoagulant Activity of Heparins In Vitro
- Authors: Drozd N.N.1, Logvinova Y.S.1, Shagdarova B.T.2, Il’ina A.V.2, Varlamov V.P.2
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Affiliations:
- National Medical Research Center of Hematology, Ministry of Health of the Russian Federation
- Institute of Bioengineering, Fundamental Basics of Biotechnology, Russian Academy of Science
- Issue: Vol 167, No 2 (2019)
- Pages: 279-283
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241645
- DOI: https://doi.org/10.1007/s10517-019-04509-w
- ID: 241645
Cite item
Abstract
Quaternized derivatives of chitosan with a substitution degree of 85-98% (highly substituted) synthesized from chitosans with a molecular weight of 5, 10, 20 kDa, with a degree of deacetylation of 89-98%, and the code numbers of QChit 5, QChit 10, QChit 20, respectively, completely neutralize antithrombin activity of unfractionated heparin and partially neutralize aXa activity of low-molecular-weight heparin (clexane), similar to protamine sulfate. The advantages of QChit 5 and QChit 10 over QChit 20 and protamine sulphate are the follows: the effect is achieved at lower concentrations and in greater concentration range; they do not promote platelet aggregation; in a concentration of 0.0072 mg/ml they do not destroy the erythrocyte membranes.
About the authors
N. N. Drozd
National Medical Research Center of Hematology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: nndrozd@mail.ru
Russian Federation, Moscow
Yu. S. Logvinova
National Medical Research Center of Hematology, Ministry of Health of the Russian Federation
Email: nndrozd@mail.ru
Russian Federation, Moscow
B. Ts. Shagdarova
Institute of Bioengineering, Fundamental Basics of Biotechnology, Russian Academy of Science
Email: nndrozd@mail.ru
Russian Federation, Moscow
A. V. Il’ina
Institute of Bioengineering, Fundamental Basics of Biotechnology, Russian Academy of Science
Email: nndrozd@mail.ru
Russian Federation, Moscow
V. P. Varlamov
Institute of Bioengineering, Fundamental Basics of Biotechnology, Russian Academy of Science
Email: nndrozd@mail.ru
Russian Federation, Moscow