Study of the Osteoindictive Properties of Protein-Modified Polylactide Scaffolds
- Authors: Nashchekina Y.A.1,2, Alexandrova S.A.1, Nikonov P.О.1, Ivankova E.I.3,4, Yudin V.E.3,4, Blinova M.I.1, Mikhailova N.A.1
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Affiliations:
- Institute of Cytology, Russian Academy of Sciences
- A. F. Ioffe Physico-Technical Institute, Russian Academy of Sciences
- Peter the Great St. Petersburg State Polytechnic University
- Institute of High-Molecular Compounds, Russian Academy of Sciences
- Issue: Vol 167, No 1 (2019)
- Pages: 164-168
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/241544
- DOI: https://doi.org/10.1007/s10517-019-04483-3
- ID: 241544
Cite item
Abstract
Bone marrow mesenchymal stromal cells are multipotent and can differentiate into cells of various tissues, which determines their high importance for clinical application. We performed an in vitro study of the osteogenic potential of mesenchymal stromal cells cultured on intact polylactide scaffolds or scaffolds modified with collagen I or fibrin. Scanning electron microscopy showed that the cells formed osteogenic nodules or osteogenic nodules on both intact and fibrin-modified polylactide scaffolds. Spectrophotometric detection of alkaline phosphatase activity on days 7 and 11 showed that mesenchymal stromal cell grown on intact polylactide scaffolds and on scaffolds modified with collagen type I or fibrin more intensively synthesized alkaline phosphatase than in the control (culture plastic). This dependence increases in the presence of osteogenic differentiation factors in the medium. After long-term culturing (4 weeks), the presence of calcium deposits detected by alizarin red staining confirmed the osteoinductive properties of intact and protein-modified polylactide scaffolds. These findings suggest that polylactide scaffolds and collagen I increase the osteogenic differentiation potential of mesenchymal stromal cells.
About the authors
Yu. A. Nashchekina
Institute of Cytology, Russian Academy of Sciences; A. F. Ioffe Physico-Technical Institute, Russian Academy of Sciences
Author for correspondence.
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg; St. Petersburg
S. A. Alexandrova
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg
P. О. Nikonov
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg
E. I. Ivankova
Peter the Great St. Petersburg State Polytechnic University; Institute of High-Molecular Compounds, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg; St. Petersburg
V. E. Yudin
Peter the Great St. Petersburg State Polytechnic University; Institute of High-Molecular Compounds, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg; St. Petersburg
M. I. Blinova
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg
N. A. Mikhailova
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg