Development of an In Vitro Model of Inflammatory Cytokine Influences on Intervertebral Disk Cells in 3D Cell Culture Using Activated Macrophage-Like THP-1 Cells
- Authors: Byvaltsev V.A.1,2,3, Kolesnikov S.I.4,5, Bardonova L.A.1,6, Belykh E.G1,6, Korytov L.I.1, Giers M.B.7,6, Bowen S.6, Preul M.C.6
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Affiliations:
- Irkutsk State Medical University
- Irkutsk Research Center of Surgery and Traumatology
- Railroad Clinical Hospital at Irkutsk-Passenger Station, Russian Railways Company
- Research Center for Problems of Family Health and Human Reproduction
- M. V. Lomonosov Moscow State University
- Barrow Neurological Institute
- Oregon State University
- Issue: Vol 166, No 1 (2018)
- Pages: 151-154
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240877
- DOI: https://doi.org/10.1007/s10517-018-4304-6
- ID: 240877
Cite item
Abstract
We developed a new model for evaluation of the influence of proinflammatory cytokines on intervertebral disc cells in a 3D culture based on co-culturing of these cells with activated macrophage-like THP-1 cells. The levels of TNFα, IL-1β, IL-6, IL-8, IL-10, and IL-12p70 production were assessed by flow cytofluorometry using microspheres. Considerable differences in the level of spontaneous cytokine secretion by normal and degenerated intervertebral disc cells were revealed. A significant increase in the level of IL-1β and IL-8 was observed during co-culturing, which confirms consistency of the developed model.
About the authors
V. A. Byvaltsev
Irkutsk State Medical University; Irkutsk Research Center of Surgery and Traumatology; Railroad Clinical Hospital at Irkutsk-Passenger Station, Russian Railways Company
Author for correspondence.
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Irkutsk; Irkutsk
S. I. Kolesnikov
Research Center for Problems of Family Health and Human Reproduction; M. V. Lomonosov Moscow State University
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Moscow
L. A. Bardonova
Irkutsk State Medical University; Barrow Neurological Institute
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Phoenix, AZ
E. G Belykh
Irkutsk State Medical University; Barrow Neurological Institute
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Phoenix, AZ
L. I. Korytov
Irkutsk State Medical University
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk
M. B. Giers
Oregon State University; Barrow Neurological Institute
Email: byval75vadim@yandex.ru
United States, Corvallis, OR; Phoenix, AZ
S. Bowen
Barrow Neurological Institute
Email: byval75vadim@yandex.ru
United States, Phoenix, AZ
M. C. Preul
Barrow Neurological Institute
Email: byval75vadim@yandex.ru
United States, Phoenix, AZ