Human Umbilical Cord Mesenchymal Stromal Cell-Derived Microvesicles Express Surface Markers Identical to the Phenotype of Parental Cells
- Authors: Romanov Y.A.1,2, Volgina N.E.3, Dugina T.N.2, Kabaeva N.V.1, Sukhikh G.T.3
-
Affiliations:
- National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
- CryoCenter Cord Blood Bank
- V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Issue: Vol 166, No 1 (2018)
- Pages: 124-129
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/240866
- DOI: https://doi.org/10.1007/s10517-018-4300-x
- ID: 240866
Cite item
Abstract
Production of microvesicles in culture of human umbilical cord multipotent mesenchymal stromal cells was studied and comparative analysis of the expression of some surface molecules (clusters of differentiation, CD) was performed. It was found that the mesenchymal stromal cells produce microvesicles in the amount sufficient for their detection by flow cytometry. Parallel analysis of the phenotypes of maternal mesenchymal stromal cells and secreted microvesicles revealed identical expression of surface molecules CD13, CD29, CD44, CD54, CD71, CD73, CD90, CD105, CD106, and HLA-I. The concentration of microvesicles in the conditioned medium was 17.9±4.6×106/ml; i.e. one cell produced ~40-50 (44.7±11.5) microvesicles over 2 days in culture.
About the authors
Yu. A. Romanov
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation; CryoCenter Cord Blood Bank
Author for correspondence.
Email: romanov@cryocenter.ru
Russian Federation, Moscow; Moscow
N. E. Volgina
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cryocenter.ru
Russian Federation, Moscow
T. N. Dugina
CryoCenter Cord Blood Bank
Email: romanov@cryocenter.ru
Russian Federation, Moscow
N. V. Kabaeva
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
Email: romanov@cryocenter.ru
Russian Federation, Moscow
G. T. Sukhikh
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cryocenter.ru
Russian Federation, Moscow