Insulin Increases Viability of Neurons in Rat Cerebral Cortex and Normalizes Bax/Bcl-2 Ratio under Conditions of Oxidative Stress
- Authors: Bayunova L.V.1, Zorina I.I.1, Zakharova I.O.1, Avrova N.F.1
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Affiliations:
- Laboratory of Molecular Endocrinology and Neurochemistry, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences
- Issue: Vol 165, No 1 (2018)
- Pages: 14-17
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239936
- DOI: https://doi.org/10.1007/s10517-018-4088-8
- ID: 239936
Cite item
Abstract
We studied the protective effect of insulin in various concentrations and its effect on the Bax/ Bcl-2 ratio in neurons of rat cerebral cortex under conditions of oxidative stress. The protective effect of insulin was dose-dependent within the nanomolar range (1 nM<10 nM<100 nM). Preincubation with insulin in concentrations of 100 nM and 1 μM significantly increased Bcl-2 content in neurons in 5, 30, and 45 min and 1, 2, and 4 h after the start of cell exposure to H2O2. This prooxidant increased the Bax/Bcl-2 ratio in neurons to 141-164% in comparison with the control (100%); preincubation of neurons with insulin returned this ratio to normal.
About the authors
L. V. Bayunova
Laboratory of Molecular Endocrinology and Neurochemistry, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences
Email: avrova@iephb.ru
Russian Federation, St. Petersburg
I. I. Zorina
Laboratory of Molecular Endocrinology and Neurochemistry, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences
Email: avrova@iephb.ru
Russian Federation, St. Petersburg
I. O. Zakharova
Laboratory of Molecular Endocrinology and Neurochemistry, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences
Email: avrova@iephb.ru
Russian Federation, St. Petersburg
N. F. Avrova
Laboratory of Molecular Endocrinology and Neurochemistry, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences
Author for correspondence.
Email: avrova@iephb.ru
Russian Federation, St. Petersburg