Modeling of 3D Structure of Chimeric Constructs Based on Hemagglutinin of Influenza Virus and Immunogenic Epitopes of Streptococcus Agalactiae
- Authors: Fedorova E.A.1, Smolonogina T.A.1, Isakova-Sivak I.N.1, Koren’kov D.A.1, Kotomina T.S.1, Leont’eva G.F.2, Suvorov A.N.2, Rudenko L.G.1
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Affiliations:
- A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
- Department of Molecular Microbiology, Institute of Experimental Medicine
- Issue: Vol 164, No 6 (2018)
- Pages: 743-748
- Section: Virology
- URL: https://journals.rcsi.science/0007-4888/article/view/239890
- DOI: https://doi.org/10.1007/s10517-018-4071-4
- ID: 239890
Cite item
Abstract
A project of an experimental recombinant vector vaccine for prevention of diseases caused by pathogenic streptococci based on ScaAB lipoprotein of Streptococcus agalactiae and a coldadapted strain of live influenza vaccine as a vector was developed. The sequence of ScaAB lipoprotein was analyzed and fragments forming immunodominant epitopes were determined. Chimeric molecules of influenza virus hemagglutinin H7 carrying insertions of bacterial origin were constructed. Based on the results of simulation, the most promising variants were selected; they represented fragments of lipoprotein ScaAB lacking N-terminal domain bound to hemagglutinin via a flexible linker. These insertions should minimally modulate the properties of the influenza strain, while retaining potential immunogenicity to a wide group of pathogenic streptococci.
About the authors
E. A. Fedorova
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Author for correspondence.
Email: fedorova.iem@gmail.com
Russian Federation, St. Petersburg
T. A. Smolonogina
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: fedorova.iem@gmail.com
Russian Federation, St. Petersburg
I. N. Isakova-Sivak
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: fedorova.iem@gmail.com
Russian Federation, St. Petersburg
D. A. Koren’kov
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: fedorova.iem@gmail.com
Russian Federation, St. Petersburg
T. S. Kotomina
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: fedorova.iem@gmail.com
Russian Federation, St. Petersburg
G. F. Leont’eva
Department of Molecular Microbiology, Institute of Experimental Medicine
Email: fedorova.iem@gmail.com
Russian Federation, St. Petersburg
A. N. Suvorov
Department of Molecular Microbiology, Institute of Experimental Medicine
Email: fedorova.iem@gmail.com
Russian Federation, St. Petersburg
L. G. Rudenko
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: fedorova.iem@gmail.com
Russian Federation, St. Petersburg