Evaluation of the Efficiency of Lytic Mycobacteriophage D29 on the Model of M. tuberculosis-Infected Macrophage RAW 264 Cell Line
- Authors: Lapenkova M.B.1, Smirnova N.S.1, Rutkevich P.N.2, Vladimirsky M.A.1
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Affiliations:
- Research Institute of Phthisiopulmonology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
- Research Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex, Ministry of Health of the Russian Federation
- Issue: Vol 164, No 3 (2018)
- Pages: 344-346
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239589
- DOI: https://doi.org/10.1007/s10517-018-3986-0
- ID: 239589
Cite item
Abstract
Culture of mouse macrophages (RAW 264.7 ATCC strain) in wells of a 6-well plate was infected with M. tuberculosis in proportion of 15 mycobacteria per one macrophage and then treated with a lytic strain of mycobacteriophage D29. Antibacterial efficacy of mycobacteriophages was studied using D29 phage (activity 108 plaque-forming units/ml) previously purified by ion exchange chromatography. After single and double 24-h treatment, the lysed cultures of macrophages were inoculated onto Middlebrook 7H10 agar medium. The number of mycobacterial colonies in control and test wells (at least 3 wells in each group) was 300.178±12.500 and 36.0±5.4, respectively (p<0.01).
About the authors
M. B. Lapenkova
Research Institute of Phthisiopulmonology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: mvladimirskij@mail.ru
Russian Federation, Moscow
N. S. Smirnova
Research Institute of Phthisiopulmonology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: mvladimirskij@mail.ru
Russian Federation, Moscow
P. N. Rutkevich
Research Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex, Ministry of Health of the Russian Federation
Email: mvladimirskij@mail.ru
Russian Federation, Moscow
M. A. Vladimirsky
Research Institute of Phthisiopulmonology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Author for correspondence.
Email: mvladimirskij@mail.ru
Russian Federation, Moscow