Morphological Changes in the Thymus, Composition of Its Cells, and Subpopulations of Peripheral Blood Lymphocytes during Experimental Acute Ulcerative Colitis
- Authors: Makarova O.V.1,2, Postovalova E.A.1,2
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Affiliations:
- Research Institute of Human Morphology, M. V. Lomonosov Moscow State University
- Department of Cell Biology and Histology, Faculty of Biology, M. V. Lomonosov Moscow State University
- Issue: Vol 163, No 5 (2017)
- Pages: 681-686
- Section: Morphology and Pathomorphology
- URL: https://journals.rcsi.science/0007-4888/article/view/239190
- DOI: https://doi.org/10.1007/s10517-017-3878-8
- ID: 239190
Cite item
Abstract
Morphological changes in the thymus and composition of its cells and peripheral blood lymphocytes were studied in experiments on C57Bl/6 mice with sodium dextran sulfate-induced acute ulcerative colitis. Severe acute ulcerative colitis in rats was accompanied by stage III-IV accidental involution of the thymus. This state was characterized by inversion of the layers, death of thymocytes, and increase in the number and area of thymic corpuscles from CK19+ epithelial cells. Flow cytofluorometry revealed an increase in the relative number of F4/80+ macrophages in the thymus stroma and CD4—CD8—CD45+CD11c+ dendritic cells and CD326+UEA+CD205— epithelial cells in the medulla. By contrast, the count of CD326+UEA—CD205+ epithelial cells remained unchanged in the cortex. Accidental involution of the thymus was accompanied by an increase in the number of apoptotic AnnV+PI— cells, but decrease in the count of lymphocytes, CD3—CD19+ B lymphocytes, CD3+CD8+ cytotoxic T lymphocytes, immature CD4+CD8+ lymphocytes, and CD3+CD4+ T helpers. The level of peripheral blood endotoxin in adult male C57Bl/6 mice with fibrinous ulcerative colitis was 10-fold lower than in the control. Moreover, we observed a decrease in the absolute number of leukocytes, lymphocytes, CD3+CD4+ T helpers, CD3+CD8+ cytotoxic T lymphocytes, CD4+CD25+FOXP3+ regulatory T lymphocytes, and CD3—CD19+ B lymphocytes in the peripheral blood of animals.
About the authors
O. V. Makarova
Research Institute of Human Morphology, M. V. Lomonosov Moscow State University; Department of Cell Biology and Histology, Faculty of Biology, M. V. Lomonosov Moscow State University
Email: e.a.postovalova@gmail.com
Russian Federation, Moscow; Moscow
E. A. Postovalova
Research Institute of Human Morphology, M. V. Lomonosov Moscow State University; Department of Cell Biology and Histology, Faculty of Biology, M. V. Lomonosov Moscow State University
Author for correspondence.
Email: e.a.postovalova@gmail.com
Russian Federation, Moscow; Moscow