hsa-miR-1973 MicroRNA is Significantly and Differentially Expressed in MDA-MB-231 Cells of Breast Adenocarcinoma and Xenografts Derived from the Tumor
- Authors: Fomicheva K.A.1, Knyazev E.N.1,2, Mal’tseva D.V.2
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Affiliations:
- P. A. Herzen Moscow Oncology Research Center — Branch of National Medical Radiological Research Center, Ministry of Health of the Russian Federation
- BioClinicum Research Center
- Issue: Vol 163, No 5 (2017)
- Pages: 660-662
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239168
- DOI: https://doi.org/10.1007/s10517-017-3873-0
- ID: 239168
Cite item
Abstract
The gene expression profiles of MDA-MB-231 cell line of breast cancer and xenografts derived from this tumor in murine model were analyzed using GeneChip Human Transcriptome array 2.0 platform (Affymetrix). A more than 1000-fold increase in the MIR1973 gene expression was observed in the xenografts compared to the original cell line. Real-time reverse transcription PCR showed that the content of mature hsa-miR-1973 microRNA encoded by this gene was elevated in the xenografts by more than 300 times. According to microarray analysis, none of hsa-miR-1973 target genes available in the databases changed the expression in the cell line and xenografts. A possible role of hsa-miR-1973 is reduction of apoptosis in the tumor.
About the authors
K. A. Fomicheva
P. A. Herzen Moscow Oncology Research Center — Branch of National Medical Radiological Research Center, Ministry of Health of the Russian Federation
Author for correspondence.
Email: karina-fomicheva@yandex.ru
Russian Federation, Moscow
E. N. Knyazev
P. A. Herzen Moscow Oncology Research Center — Branch of National Medical Radiological Research Center, Ministry of Health of the Russian Federation; BioClinicum Research Center
Email: karina-fomicheva@yandex.ru
Russian Federation, Moscow; Moscow
D. V. Mal’tseva
BioClinicum Research Center
Email: karina-fomicheva@yandex.ru
Russian Federation, Moscow