Association between the Prevalence of Somatic Mutations in PIK3CA Gene in Tumors and Clinical and Morphological Characteristics of Breast Cancer Patients
- Authors: Filipenko M.L.1,2, Os’kina N.A.1, Oskorbin I.A.1,2, Mishukova O.V.1,2, Ovchinnikova L.K.3, Gershtein E.S.3, Kushlinskii N.E.3
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Affiliations:
- Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
- Novosibirsk National Research State University
- N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
- Issue: Vol 163, No 2 (2017)
- Pages: 250-254
- Section: Oncology
- URL: https://journals.rcsi.science/0007-4888/article/view/238792
- DOI: https://doi.org/10.1007/s10517-017-3777-z
- ID: 238792
Cite item
Abstract
The presence of activating somatic mutations in codons 542 and 545 of exon 9 (p.E542K c.1624G>A and p.E545K c.1633G>A) and in codon 1047 of exon 20 (p.H1047R c.3140A>G and p.H1047L c.3140A>T) of PIK3CA gene encoding catalytic p110α-subunit of phosphatidylinositol-3-kinase was studied in tumors of 473 breast cancer patients by multiplex allele-specific real-time PCR. Fifty-eight (12.3%) different mutations were found. An increase in the frequency of PIK3CA gene mutations with disease progression (from 2.4 to 28.7% with tumor progression from I-IIa to III-IV stage; p=0.0001) and a trend towards its increase in the tumors with unfavorable prognostic characteristics (high histological grade, triple negative phenotype) were demonstrated. The presence of the studied PIK3CA gene mutations in tumors significantly reduces relapse-free survival in the total group and in stage III cancer patients.
About the authors
M. L. Filipenko
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences; Novosibirsk National Research State University
Author for correspondence.
Email: biochimia@yandex.ru
Russian Federation, Novosibirsk; Novosibirsk
N. A. Os’kina
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: biochimia@yandex.ru
Russian Federation, Novosibirsk
I. A. Oskorbin
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences; Novosibirsk National Research State University
Email: biochimia@yandex.ru
Russian Federation, Novosibirsk; Novosibirsk
O. V. Mishukova
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences; Novosibirsk National Research State University
Email: biochimia@yandex.ru
Russian Federation, Novosibirsk; Novosibirsk
L. K. Ovchinnikova
N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
Email: biochimia@yandex.ru
Russian Federation, Moscow
E. S. Gershtein
N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
Email: biochimia@yandex.ru
Russian Federation, Moscow
N. E. Kushlinskii
N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
Email: biochimia@yandex.ru
Russian Federation, Moscow