Effects of Secondary Metabolites of Permafrost Bacillus sp. on Cytokine Synthesis by Human Peripheral Blood Mononuclear Cells
- Authors: Kalenova L.F.1,2, Kolyvanova S.S.1,2, Bazhin A.S.2, Besedin I.M.2, Mel’nikov V.P.1,2
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Affiliations:
- Tyumen State University
- Tyumen Research Center, Siberian Division of the Russian Academy of Sciences
- Issue: Vol 163, No 2 (2017)
- Pages: 235-238
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238780
- DOI: https://doi.org/10.1007/s10517-017-3774-2
- ID: 238780
Cite item
Abstract
We studied the effects of secondary metabolites of Bacillus sp. isolated from late Neogene permafrost on secretion of proinflammatory (TNF-α, IL-1β, IL-8, IL-2, and IFNγ) and antiinflammatory (IL-4 and IL-10) cytokines by human peripheral blood mononuclear cells. It was found that metabolites of Bacillus sp. produced more potent effect on cytokine secretion than mitogen phytohemagglutinin and metabolites of Bacillus cereus, medicinal strain IP5832. Activity of metabolites depended on the temperature of bacteria incubation. “Cold” metabolites of Bacillus sp. (isolated at -5°C) primarily induced Th1-mediated secretion of IFNγ, while “warm” metabolites (obtained at 37°C) induced Th2-mediated secretion of IL-4. The results suggest that Bacillus sp. metabolites are promising material for the development of immunomodulating drugs.
About the authors
L. F. Kalenova
Tyumen State University; Tyumen Research Center, Siberian Division of the Russian Academy of Sciences
Author for correspondence.
Email: lkalenova@mail.ru
Russian Federation, Tyumen; Tyumen
S. S. Kolyvanova
Tyumen State University; Tyumen Research Center, Siberian Division of the Russian Academy of Sciences
Email: lkalenova@mail.ru
Russian Federation, Tyumen; Tyumen
A. S. Bazhin
Tyumen Research Center, Siberian Division of the Russian Academy of Sciences
Email: lkalenova@mail.ru
Russian Federation, Tyumen
I. M. Besedin
Tyumen Research Center, Siberian Division of the Russian Academy of Sciences
Email: lkalenova@mail.ru
Russian Federation, Tyumen
V. P. Mel’nikov
Tyumen State University; Tyumen Research Center, Siberian Division of the Russian Academy of Sciences
Email: lkalenova@mail.ru
Russian Federation, Tyumen; Tyumen