Effect of Phenibut and Glufimet, a Novel Glutamic Acid Derivative, on Respiration of Heart and Brain Mitochondria from Animals Exposed to Stress against the Background of Inducible NO-Synthase Blockade
- Authors: Perfilova V.N.1, Popova T.A.2, Prokofiev I.I.1, Mokrousov I.S.1, Ostrovskii O.V.2, Tyurenkov I.N.1
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Affiliations:
- Department of Pharmacology and Biopharmaceutics Doctor Improvement Faculty
- Department of Theoretical Biochemistry with a Course of Clinical Biochemistry, Volgograd State Medical University
- Issue: Vol 163, No 2 (2017)
- Pages: 226-229
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238773
- DOI: https://doi.org/10.1007/s10517-017-3772-4
- ID: 238773
Cite item
Abstract
Increased oxygen consumption by heart and brain mitochondria in the absence of ADP and reduced mitochondrial respiration in the presence of ADP were observed in rats exposed to stress simulated by suspension by the dorsal neck skin fold for 24 h, which attests to uncoupling of substrate oxidation and ATP synthesis and can cause electron drain from the respiratory chain, formation of ROS, and oxidative damage to cell structures. Blockade of inducible NO synthase with aminoguanidine (single intraperitoneal dose of 50 mg/kg before stress exposure) increased coupling of respiration and oxidative phosphorylation in heart and brain mitochondria of rats exposed to immobilization-painful stress, which was especially pronounced in cardiomyocytes. The test compounds glufimet (single intraperitoneal dose of 29 mg/kg before stress exposure) and phenibut (single intraperitoneal dose of 50 mg/kg before stress exposure) limited stress-induced mitochondrial damage against the background of inducible NO synthase blockade and without it, which was seen from increased respiratory control ratio in comparison with that in untreated rats exposed to stress (control).
About the authors
V. N. Perfilova
Department of Pharmacology and Biopharmaceutics Doctor Improvement Faculty
Email: igor.prokofiev@mail.ru
Russian Federation, Volgograd
T. A. Popova
Department of Theoretical Biochemistry with a Course of Clinical Biochemistry, Volgograd State Medical University
Email: igor.prokofiev@mail.ru
Russian Federation, Volgograd
I. I. Prokofiev
Department of Pharmacology and Biopharmaceutics Doctor Improvement Faculty
Author for correspondence.
Email: igor.prokofiev@mail.ru
Russian Federation, Volgograd
I. S. Mokrousov
Department of Pharmacology and Biopharmaceutics Doctor Improvement Faculty
Email: igor.prokofiev@mail.ru
Russian Federation, Volgograd
O. V. Ostrovskii
Department of Theoretical Biochemistry with a Course of Clinical Biochemistry, Volgograd State Medical University
Email: igor.prokofiev@mail.ru
Russian Federation, Volgograd
I. N. Tyurenkov
Department of Pharmacology and Biopharmaceutics Doctor Improvement Faculty
Email: igor.prokofiev@mail.ru
Russian Federation, Volgograd