Local and Systemic Functional Responses of Mouse Macrophages to Intravaginal Infection with Type 2 Herpes Simplex Virus and Vaccination
- Authors: Zaitseva L.G.1, Bekhalo V.A.1, Kireeva I.V.1, Shaposhnikova G.M.1, Nagurskaya E.V.1, Barinskii I.F.1, Nesterenko V.G.1
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Affiliations:
- N. F. Gamaleya Federal Research Centre Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation
- Issue: Vol 163, No 1 (2017)
- Pages: 68-72
- Section: Virology
- URL: https://journals.rcsi.science/0007-4888/article/view/238653
- DOI: https://doi.org/10.1007/s10517-017-3740-z
- ID: 238653
Cite item
Abstract
Activity of cathepsin D and phagocytosis of macrophages from vaginal lavage fluid, peritoneal exudation, and spleen were studied in mice of sensitive (DBA/2) and resistant (BALB/c) lines after intravaginal infection with type 2 herpes simplex virus and vaccination. Activity of cathepsin D and intensity of phagocytosis (irrespective of the macrophage source) and their ratio in BALB/c mice in early terms after infection were close to the control levels taken as a unit. In DBA/2 mice, these parameters and their balance were shifted and changes in cathepsin D activity depended on the time after challenge. Activities of cellular and extracellular cathepsin D increased sharply on day 1 postinfection under conditions of local virus interaction with the vaginal mucosa and activation of the pathological process. Later, after generalization of the infection, activity of cathepsin D decreased, while phagocytosis increased in all the studied macrophage populations. Vaccination corrected the cathepsin D/phagocytosis imbalance and created conditions for rapid elimination of the virus.
About the authors
L. G. Zaitseva
N. F. Gamaleya Federal Research Centre Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation
Email: elenarealnm@gmail.com
Russian Federation, Moscow
V. A. Bekhalo
N. F. Gamaleya Federal Research Centre Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation
Email: elenarealnm@gmail.com
Russian Federation, Moscow
I. V. Kireeva
N. F. Gamaleya Federal Research Centre Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation
Email: elenarealnm@gmail.com
Russian Federation, Moscow
G. M. Shaposhnikova
N. F. Gamaleya Federal Research Centre Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation
Email: elenarealnm@gmail.com
Russian Federation, Moscow
E. V. Nagurskaya
N. F. Gamaleya Federal Research Centre Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation
Author for correspondence.
Email: elenarealnm@gmail.com
Russian Federation, Moscow
I. F. Barinskii
N. F. Gamaleya Federal Research Centre Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation
Email: elenarealnm@gmail.com
Russian Federation, Moscow
V. G. Nesterenko
N. F. Gamaleya Federal Research Centre Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation
Email: elenarealnm@gmail.com
Russian Federation, Moscow