Role of Methylation in the Regulation of Apoptosis Genes APAF1, DAPK1, and BCL2 in Breast Cancer
- Authors: Loginov V.I.1, Pronina I.V.2, Burdennyi A.M.1, Pereyaslova E.A.1, Braga E.A.1, Kazubskaya T.P.3, Kushlinskii N.E.3
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Affiliations:
- Research Institute of Pathology and Pathophysiology
- Medical Genetic Research Center
- N. N. Blokhin Russian Cancer Research Centre, The Ministry of Health of the Russian Federation
- Issue: Vol 162, No 6 (2017)
- Pages: 797-800
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238579
- DOI: https://doi.org/10.1007/s10517-017-3716-z
- ID: 238579
Cite item
Abstract
Changes in the levels of expression of proapoptotic genes APAF1 and DAPK1 and antiapoptotic gene BCL2 were studied by real time PCR in specimens of tumors and histologically intact tissue from 28 patients with breast cancer. The expression of APAF1 and DAPK1 was below the normal in the majority of tumor samples (p<0.05), while the level of BCL2 mRNA more often surpassed the normal (p<0.1). Study of the same sample of specimens by methylspecific PCR showed predominance of APAF1 and DAPK1 hypermethylation (p<0.05 and p<0.1, respectively) and more frequent hypomethylation of BCL2. A significant correlation between changes in the levels of expression and methylation (r=0.40-0.49; p<0.05) was detected for all three genes (APAF1, DAPK1, and BCL2). The results suggest that methylation play an important role in the regulation of these apoptosis system genes in breast cancer
About the authors
V. I. Loginov
Research Institute of Pathology and Pathophysiology
Author for correspondence.
Email: loginov7w@gmail.com
Russian Federation, Moscow
I. V. Pronina
Medical Genetic Research Center
Email: loginov7w@gmail.com
Russian Federation, Moscow
A. M. Burdennyi
Research Institute of Pathology and Pathophysiology
Email: loginov7w@gmail.com
Russian Federation, Moscow
E. A. Pereyaslova
Research Institute of Pathology and Pathophysiology
Email: loginov7w@gmail.com
Russian Federation, Moscow
E. A. Braga
Research Institute of Pathology and Pathophysiology
Email: loginov7w@gmail.com
Russian Federation, Moscow
T. P. Kazubskaya
N. N. Blokhin Russian Cancer Research Centre, The Ministry of Health of the Russian Federation
Email: loginov7w@gmail.com
Russian Federation, Moscow
N. E. Kushlinskii
N. N. Blokhin Russian Cancer Research Centre, The Ministry of Health of the Russian Federation
Email: loginov7w@gmail.com
Russian Federation, Moscow