Conditions for Collection of Placental Tissue Samples for Culturing of Multipotent Mesenchymal Stromal Cells
- Authors: Nizyaeva N.V.1, Nagovitsyna M.N.1, Kulikova G.V.1, Tumanova U.N.1, Poltavtseva R.A.1, Fedorova I.A.1, Yushina M.N.1, Pavlovich S.V.1, Shchyogolev A.I.1
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Affiliations:
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
- Issue: Vol 162, No 4 (2017)
- Pages: 501-506
- Section: Morphology and Pathomorphology
- URL: https://journals.rcsi.science/0007-4888/article/view/238323
- DOI: https://doi.org/10.1007/s10517-017-3648-7
- ID: 238323
Cite item
Abstract
Placentas from women aged 25-32 years with normal course of gestation were studied. It is essential to stick to certain methodological approaches for preparing viable multipotent mesenchymal stromal cell culture and to carry out morphological (macro and micro) evaluation of the chorionic villi, umbilical cords, and placentas. At stage I of the study, patients’ histories, labor course, and examinations of the newborns should be analyzed to exclude women with genital and extragenital diseases. At stage II, it is essential to stick to special regulations and methods for collection of specimens of the cord, amnion, and placental tissue proper. Histological control of the placental structures collected for multipotent mesenchymal stromal cell culturing is obligatory.
About the authors
N. V. Nizyaeva
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
Email: ashegolev@oparina4.ru
Russian Federation, Moscow
M. N. Nagovitsyna
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
Email: ashegolev@oparina4.ru
Russian Federation, Moscow
G. V. Kulikova
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
Email: ashegolev@oparina4.ru
Russian Federation, Moscow
U. N. Tumanova
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
Email: ashegolev@oparina4.ru
Russian Federation, Moscow
R. A. Poltavtseva
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
Email: ashegolev@oparina4.ru
Russian Federation, Moscow
I. A. Fedorova
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
Email: ashegolev@oparina4.ru
Russian Federation, Moscow
M. N. Yushina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
Email: ashegolev@oparina4.ru
Russian Federation, Moscow
S. V. Pavlovich
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
Email: ashegolev@oparina4.ru
Russian Federation, Moscow
A. I. Shchyogolev
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation
Author for correspondence.
Email: ashegolev@oparina4.ru
Russian Federation, Moscow