Analysis of Immune Epitopes of Respiratory Syncytial Virus for Designing of Vectored Vaccines Based on Influenza Virus Platform
- Authors: Isakova-Sivak I.N.1, Korenkov D.A.1, Fedorova E.A.1, Tretiak T.S.1, Matyushenko V.A.1, Smolonogina T.A.1, Rudenko L.G.1
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Affiliations:
- A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
- Issue: Vol 161, No 4 (2016)
- Pages: 533-537
- Section: Virology
- URL: https://journals.rcsi.science/0007-4888/article/view/237574
- DOI: https://doi.org/10.1007/s10517-016-3454-7
- ID: 237574
Cite item
Abstract
The immunoepitope database was used for analysis of experimentally detected epitopes of the respiratory syncytial virus (RSV) proteins and for selection of the epitope combinations for subsequent designing of recombinant vectored anti-RSV vaccines based on attenuated influenza viruses. Three cassettes containing the most promising B- and T-cell RSV epitopes were selected: peptide F (243-294) supporting the formation of humoral immunity in animals; fragment M2-1 (70-101+114-146) containing two MHC I epitopes (82-90 and 127-135); and MHC II-epitope (51-66). The selected constructions contained no neoepitopes causing undesirable effects of vaccination, such as immunotolerance or autoimmunity.
About the authors
I. N. Isakova-Sivak
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Author for correspondence.
Email: isakova.sivak@gmail.com
Russian Federation, St. Petersburg
D. A. Korenkov
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: isakova.sivak@gmail.com
Russian Federation, St. Petersburg
E. A. Fedorova
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: isakova.sivak@gmail.com
Russian Federation, St. Petersburg
T. S. Tretiak
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: isakova.sivak@gmail.com
Russian Federation, St. Petersburg
V. A. Matyushenko
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: isakova.sivak@gmail.com
Russian Federation, St. Petersburg
T. A. Smolonogina
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: isakova.sivak@gmail.com
Russian Federation, St. Petersburg
L. G. Rudenko
A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine
Email: isakova.sivak@gmail.com
Russian Federation, St. Petersburg