A New Chemiluminescent Method for Evaluation of the Functional Activity of Neutrophils in Patients with Type 2 Diabetes Mellitus
- Authors: Proskurnina E.V.1, Sozarukova M.M.1, Polimova A.M.1, Prudnikova M.A.2, Ametov A.S.2, Vladimirov Y.A.1
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Affiliations:
- Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University
- Russian Medical Academy of Postgraduate Education
- Issue: Vol 161, No 2 (2016)
- Pages: 320-322
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237389
- DOI: https://doi.org/10.1007/s10517-016-3405-3
- ID: 237389
Cite item
Abstract
Functional activity of neutrophils was evaluated by the chemiluminescent method with successive double stimulation by soluble stimuli with different mechanisms of action: phorbol-12-myristate-13-acetate (PMA) and phormyl-methionyl-leucyl-phenilalanine (fMLP). The study was carried out in 26 patients receiving oral sugar-reducing therapy. In addition to the functional activity of neutrophils, the levels of TBA reactive products, inflammation markers, blood clotting values, and biochemical parameters were measured. The results showed mainly reduction of the granulocytic component of the immune system in the patients.
About the authors
E. V. Proskurnina
Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University
Author for correspondence.
Email: proskurnina@gmail.com
Russian Federation, Moscow
M. M. Sozarukova
Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University
Email: proskurnina@gmail.com
Russian Federation, Moscow
A. M. Polimova
Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University
Email: proskurnina@gmail.com
Russian Federation, Moscow
M. A. Prudnikova
Russian Medical Academy of Postgraduate Education
Email: proskurnina@gmail.com
Russian Federation, Moscow
A. S. Ametov
Russian Medical Academy of Postgraduate Education
Email: proskurnina@gmail.com
Russian Federation, Moscow
Yu. A. Vladimirov
Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University
Email: proskurnina@gmail.com
Russian Federation, Moscow