Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from Human Endometrium
- Authors: Savilova A.M.1, Yushina M.N.1, Rudimova Y.V.1, Khil’kevich E.G.1, Chuprynin V.D.1
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Affiliations:
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Issue: Vol 160, No 4 (2016)
- Pages: 560-564
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236790
- DOI: https://doi.org/10.1007/s10517-016-3218-4
- ID: 236790
Cite item
Abstract
Cell cultures isolated from human endometrium by enzyme digestion consisted of highly viable fibroblast-like mesenchymal cells expressing CD90, CD73, and CD105. During passage 1, the cultures contained a small fraction of cytokeratin-7+ epithelial cells that disappeared by passage 2. The cultures from the endometrium could be induced to adipogenic, osteogenic and chondrogenic differentiation in vitro. These findings suggest that human endometrium is a convenient source of biomaterial for minimally invasive isolation of cultures that exhibit typical morphology and immunophenotypic profile of resident multipotent mesenchymal stromal cells retain high viability in vitro.
About the authors
A. M. Savilova
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: bushueva@gmail.com
Russian Federation, Moscow
M. N. Yushina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: bushueva@gmail.com
Russian Federation, Moscow
Y. V. Rudimova
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: bushueva@gmail.com
Russian Federation, Moscow
E. G. Khil’kevich
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: bushueva@gmail.com
Russian Federation, Moscow
V. D. Chuprynin
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: bushueva@gmail.com
Russian Federation, Moscow