The Mechanism of Adaptation of Breast Cancer Cells to Hypoxia: Role of AMPK/mTOR Signaling Pathway
- Authors: Sorokin D.V.1, Scherbakov A.M.1, Yakushina I.A.1, Semina S.E.1, Gudkova M.V.1, Krasil’nikov M.A.1
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Affiliations:
- N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
- Issue: Vol 160, No 4 (2016)
- Pages: 555-559
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236787
- DOI: https://doi.org/10.1007/s10517-016-3217-5
- ID: 236787
Cite item
Abstract
We studied the mechanisms of adaptation of human breast cancer cells MCF-7 to hypoxia and analyzed the role of AMPK/mTOR signaling pathway in the maintenance of cell proliferation under hypoxic conditions. It was found that long-term culturing (30 days or more) of MCF-7 cells under hypoxic conditions induced their partial adaptation to hypoxia. Cell adaptation to hypoxia was associated with attenuation of hypoxia-dependent AMPK induction with simultaneous constitutive activation of mTOR and Akt. These findings suggest that these proteins can be promising targets for targeted therapy of tumors developing under hypoxic conditions.
About the authors
D. V. Sorokin
N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
Email: alex.scherbakov@gmail.com
Russian Federation, Moscow
A. M. Scherbakov
N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
Author for correspondence.
Email: alex.scherbakov@gmail.com
Russian Federation, Moscow
I. A. Yakushina
N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
Email: alex.scherbakov@gmail.com
Russian Federation, Moscow
S. E. Semina
N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
Email: alex.scherbakov@gmail.com
Russian Federation, Moscow
M. V. Gudkova
N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
Email: alex.scherbakov@gmail.com
Russian Federation, Moscow
M. A. Krasil’nikov
N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation
Email: alex.scherbakov@gmail.com
Russian Federation, Moscow