Sirt1 Regulates p53 Stability and Expression of Its Target S100B during Long-Term Potentiation in Rat Hippocampus
- Authors: Lisachev P.D.1, Pustylnyak V.O.2,3, Shtark M.B.2
-
Affiliations:
- Design and Technology Institute of Computer Science, Siberian Division of the Russian Academy of Medical Science
- Research Institute of Molecular Biology and Biophysics
- Novosibirsk National Research State University
- Issue: Vol 160, No 4 (2016)
- Pages: 432-434
- Section: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/236725
- DOI: https://doi.org/10.1007/s10517-016-3189-5
- ID: 236725
Cite item
Abstract
Induction of long-term potentiation in rat hippocampus was followed by short-term activation of transcription factor p53 and its subsequent degradation. We studied the effects of EX-527 (inhibitor of deacetylase Sirt1, a negative regulator of p53) and pifi thrin-β (inhibitor of p53-dependent transcription) on the levels of p53 protein and mRNA of its target gene S100B during long-term potentiation. Pifi thrin-β limited the increase in S100B mRNA content after tetanization, which confi rmed signifi cant contribution of p53 in the regulation of S100B during long-term potentiation. EX-527 completely prevented p53 degradation and increased S100B expression induced by tetanization. Thus, Str1 regulates stability of p53 and expression of its target gene S100B in rat hippocampus during long-term potentiation.
About the authors
P. D. Lisachev
Design and Technology Institute of Computer Science, Siberian Division of the Russian Academy of Medical Science
Email: mark@niimbb.ru
Russian Federation, Moscow
V. O. Pustylnyak
Research Institute of Molecular Biology and Biophysics; Novosibirsk National Research State University
Email: mark@niimbb.ru
Russian Federation, Novosibirsk; Novosibirsk
M. B. Shtark
Research Institute of Molecular Biology and Biophysics
Author for correspondence.
Email: mark@niimbb.ru
Russian Federation, Novosibirsk