An Inducible DamID System for Profiling Interactions of Nuclear Lamina Protein Component Lamin B1 with Chromosomes in Mouse Cells
- 作者: Kozhevnikova E.1,2, Leshchenko A.1, Pindyurin A.1,2
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隶属关系:
- Institute of Molecular and Cellular Biology
- Institute of Cytology and Genetics
- 期: 卷 83, 编号 5 (2018)
- 页面: 586-594
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151660
- DOI: https://doi.org/10.1134/S0006297918050115
- ID: 151660
如何引用文章
详细
At the level of DNA organization into chromatin, there are mechanisms that define gene expression profiles in specialized cell types. Genes within chromatin regions that are located at the nuclear periphery are generally expressed at lower levels; however, the nature of this phenomenon remains unclear. These parts of chromatin interact with nuclear lamina proteins like Lamin B1 and, therefore, can be identified in a given cell type by chromatin profiling of these proteins. In this study, we created and tested a Dam Identification (DamID) system induced by Cre recombinase using Lamin B1 and mouse embryonic fibroblasts. This inducible system will help to generate genome-wide profiles of chromatin proteins in given cell types and tissues with no need to dissect tissues from organs or separate cells from tissues, which is achieved by using specific regulatory DNA elements and due to the high sensitivity of the method.
作者简介
E. Kozhevnikova
Institute of Molecular and Cellular Biology; Institute of Cytology and Genetics
编辑信件的主要联系方式.
Email: kozhevnikova@mcb.nsc.ru
俄罗斯联邦, Novosibirsk, 630090; Novosibirsk, 630090
A. Leshchenko
Institute of Molecular and Cellular Biology
Email: kozhevnikova@mcb.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
A. Pindyurin
Institute of Molecular and Cellular Biology; Institute of Cytology and Genetics
Email: kozhevnikova@mcb.nsc.ru
俄罗斯联邦, Novosibirsk, 630090; Novosibirsk, 630090