MicroRNA sponge knockdowns miR-483-5p and upregulates serum ALT/AST in transgenic mice
- 作者: Zhang Y.1, Wu D.2,3, Zhang X.2, Ma N.2, Liu Y.1
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隶属关系:
- The Second Affiliated Hospital, Department of Clinical Laboratory
- Department of Biochemistry and Molecular Biology
- The First Affiliated Hospital, Department of Laboratory Diagnostics
- 期: 卷 83, 编号 1 (2018)
- 页面: 54-59
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151569
- DOI: https://doi.org/10.1134/S0006297918010078
- ID: 151569
如何引用文章
详细
MicroRNAs are involved in many biological processes. Studying microRNA function requires genetic strategies generating loss-of-function phenotypes, especially in vivo. However, few microRNA loss-of-function models have been reported in mice. Here, we generated several transgenic mouse lines to stably and specifically knockdown miR-483-5p by overexpressing microRNA sponges from CAG promoters. The different levels of expression of microRNA sponges resulted in different levels of mature miR-483-5p, which upregulated serum ALT/AST in these transgenic lines. These results indicate microRNA sponges are effective in mice in vivo, and they can be used in microRNA loss-of-function research.
作者简介
Yanfen Zhang
The Second Affiliated Hospital, Department of Clinical Laboratory
Email: 460205012@qq.com
中国, Harbin, 150086
Dantong Wu
Department of Biochemistry and Molecular Biology; The First Affiliated Hospital, Department of Laboratory Diagnostics
Email: 460205012@qq.com
中国, Harbin, 150086; Harbin, 150086
Xuemei Zhang
Department of Biochemistry and Molecular Biology
Email: 460205012@qq.com
中国, Harbin, 150086
Ning Ma
Department of Biochemistry and Molecular Biology
Email: 460205012@qq.com
中国, Harbin, 150086
Yanhong Liu
The Second Affiliated Hospital, Department of Clinical Laboratory
编辑信件的主要联系方式.
Email: 460205012@qq.com
中国, Harbin, 150086
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