Y-box-binding protein 1 stimulates abasic site cleavage
- 作者: Alemasova E.1, Naumenko K.1,2, Moor N.1, Lavrik O.1,2
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隶属关系:
- Institute of Chemical Biology and Fundamental Medicine
- Novosibirsk State University
- 期: 卷 82, 编号 12 (2017)
- 页面: 1521-1528
- 栏目: Regular Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151541
- DOI: https://doi.org/10.1134/S0006297917120112
- ID: 151541
如何引用文章
详细
Apurinic/apyrimidinic (AP) sites are among the most frequent DNA lesions. The first step in the AP site repair involves the magnesium-dependent enzyme AP endonuclease 1 (APE1) that catalyzes hydrolytic cleavage of the DNA phosphodiester bond at the 5′ side of the AP site, thereby generating a single-strand DNA break flanked by the 3′-OH and 5′-deoxyribose phosphate (dRP) groups. Increased APE1 activity in cancer cells might correlate with tumor chemoresistance to DNA-damaging treatment. It has been previously shown that the multifunctional oncoprotein Y-box-binding protein 1 (YB-1) interacts with APE1 and inhibits APE1-catalyzed hydrolysis of AP sites in single-stranded DNAs. In this work, we demonstrated that YB-1 stabilizes the APE1 complex with double-stranded DNAs containing the AP sites and stimulates cleavage of these AP sites at low magnesium concentrations.
作者简介
E. Alemasova
Institute of Chemical Biology and Fundamental Medicine
Email: lavrik@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
K. Naumenko
Institute of Chemical Biology and Fundamental Medicine; Novosibirsk State University
Email: lavrik@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090; Novosibirsk, 630090
N. Moor
Institute of Chemical Biology and Fundamental Medicine
Email: lavrik@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
O. Lavrik
Institute of Chemical Biology and Fundamental Medicine; Novosibirsk State University
编辑信件的主要联系方式.
Email: lavrik@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090; Novosibirsk, 630090