Modern approaches for identification of modified nucleotides in RNA
- 作者: Filippova J.1, Semenov D.1, Juravlev E.1,2, Komissarov A.3,4, Richter V.1, Stepanov G.1,2
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隶属关系:
- Institute of Chemical Biology and Fundamental Medicine
- Novosibirsk State University
- Research Institute of Influenza
- St. Petersburg University
- 期: 卷 82, 编号 11 (2017)
- 页面: 1217-1233
- 栏目: Review
- URL: https://journals.rcsi.science/0006-2979/article/view/151495
- DOI: https://doi.org/10.1134/S0006297917110013
- ID: 151495
如何引用文章
详细
This review considers approaches for detection of modified monomers in the RNA structure of living organisms. Recently, some data on dynamic alterations in the pool of modifications of the key RNA species that depend on external factors affecting the cells and physiological conditions of the whole organism have been accumulated. The recent studies have presented experimental data on relationship between the mechanisms of formation of modified/minor nucleotides of RNA in mammalian cells and the development of various pathologies. The development of novel methods for detection of chemical modifications of RNA nucleotides in the cells of living organisms and accumulation of knowledge on the contribution of modified monomers to metabolism and functioning of individual RNA species establish the basis for creation of novel diagnostic and therapeutic approaches. This review includes a short description of routine methods for determination of modified nucleotides in RNA and considers in detail modern approaches that enable not only detection but also quantitative assessment of the modification level of various nucleotides in individual RNA species.
作者简介
J. Filippova
Institute of Chemical Biology and Fundamental Medicine
Email: stepanovga@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
D. Semenov
Institute of Chemical Biology and Fundamental Medicine
Email: stepanovga@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
E. Juravlev
Institute of Chemical Biology and Fundamental Medicine; Novosibirsk State University
Email: stepanovga@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090; Novosibirsk, 630090
A. Komissarov
Research Institute of Influenza; St. Petersburg University
Email: stepanovga@niboch.nsc.ru
俄罗斯联邦, St. Petersburg, 197376; St. Petersburg, 199034
V. Richter
Institute of Chemical Biology and Fundamental Medicine
Email: stepanovga@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
G. Stepanov
Institute of Chemical Biology and Fundamental Medicine; Novosibirsk State University
编辑信件的主要联系方式.
Email: stepanovga@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090; Novosibirsk, 630090
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