Interaction of cholera toxin B-subunit with human T-lymphocytes
- 作者: Navolotskaya E.1, Sadovnikov V.1, Zinchenko D.1, Zolotarev Y.2, Lipkin V.3, Zav’yalov V.4
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隶属关系:
- Branch of Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
- Institute of Molecular Genetics
- Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
- University of Turku
- 期: 卷 82, 编号 9 (2017)
- 页面: 1036-1041
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151467
- DOI: https://doi.org/10.1134/S0006297917090061
- ID: 151467
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详细
In this work, 125I-labeled cholera toxin B-subunit (CT-B) (specific activity 98 Ci/mmol) was prepared, and its high-affinity binding to human blood T-lymphocytes (Kd = 3.3 nM) was determined. The binding of the 125I-labeled CT-B was inhibited by unlabeled interferon-α2 (IFN-α2), thymosin-α1 (TM-α1), and by the synthetic peptide LKEKK, which corresponds to sequences 16-20 of human TM-α1 and 131-135 of IFN-α2 (Ki 0.8, 1.2, and 1.6 nM, respectively), but was not inhibited by the unlabeled synthetic peptide KKEKL with inverted sequence (Ki > 1 μM). In the concentration range of 10-1000 nM, both CT-B and peptide LKEKK dose-dependently increased the activity of soluble guanylate cyclase (sGC) but did not affect the activity of membrane-bound guanylate cyclase. The KKEKL peptide tested in parallel did not affect sGC activity. Thus, the CT-B and peptide LKEKK binding to a common receptor on the surface of T-lymphocytes leads to an increase in sGC activity.
作者简介
E. Navolotskaya
Branch of Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
编辑信件的主要联系方式.
Email: navolotskaya@fibkh.serpukhov.su
俄罗斯联邦, Pushchino, Moscow Region, 142290
V. Sadovnikov
Branch of Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: navolotskaya@fibkh.serpukhov.su
俄罗斯联邦, Pushchino, Moscow Region, 142290
D. Zinchenko
Branch of Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: navolotskaya@fibkh.serpukhov.su
俄罗斯联邦, Pushchino, Moscow Region, 142290
Y. Zolotarev
Institute of Molecular Genetics
Email: navolotskaya@fibkh.serpukhov.su
俄罗斯联邦, Moscow, 123182
V. Lipkin
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: navolotskaya@fibkh.serpukhov.su
俄罗斯联邦, Moscow, 117997
V. Zav’yalov
University of Turku
Email: navolotskaya@fibkh.serpukhov.su
芬兰, Turku, 20500
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