Inhibition of chaperonin GroEL by a monomer of ovine prion protein and its oligomeric forms
- 作者: Kudryavtseva S.1, Stroylova Y.1, Zanyatkin I.2, Haertle T.3, Muronetz V.1,2
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隶属关系:
- Belozersky Institute of Physico-Chemical Biology
- Lomonosov Moscow State University
- Institut National de la Recherche Agronomique
- 期: 卷 81, 编号 10 (2016)
- 页面: 1213-1220
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151063
- DOI: https://doi.org/10.1134/S0006297916100199
- ID: 151063
如何引用文章
详细
The possibility of inhibition of chaperonin functional activity by amyloid proteins was studied. It was found that the ovine prion protein PrP as well as its oligomeric and fibrillar forms are capable of binding with the chaperonin GroEL. Besides, GroEL was shown to promote amyloid aggregation of the monomeric and oligomeric PrP as well as PrP fibrils. The monomeric PrP was shown to inhibit the GroEL-assisted reactivation of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The oligomers of PrP decelerate the GroEL-assisted reactivation of GAPDH, and PrP fibrils did not affect this process. The chaperonin GroEL is capable of interacting with GAPDH and different PrP forms simultaneously. A possible role of the inhibition of chaperonins by amyloid proteins in the misfolding of the enzymes involved in cell metabolism and in progression of neurodegenerative diseases of amyloid nature is discussed.
作者简介
S. Kudryavtseva
Belozersky Institute of Physico-Chemical Biology
Email: vimuronets@belozersky.msu.ru
俄罗斯联邦, Moscow, 119234
Y. Stroylova
Belozersky Institute of Physico-Chemical Biology
Email: vimuronets@belozersky.msu.ru
俄罗斯联邦, Moscow, 119234
I. Zanyatkin
Lomonosov Moscow State University
Email: vimuronets@belozersky.msu.ru
俄罗斯联邦, Moscow, 119234
T. Haertle
Institut National de la Recherche Agronomique
Email: vimuronets@belozersky.msu.ru
法国, Nantes, 44000
V. Muronetz
Belozersky Institute of Physico-Chemical Biology; Lomonosov Moscow State University
编辑信件的主要联系方式.
Email: vimuronets@belozersky.msu.ru
俄罗斯联邦, Moscow, 119234; Moscow, 119234