Free initiation factors eIF4A and eIF4B are dispensable for translation initiation on uncapped mRNAs
- 作者: Sakharov P.1, Agalarov S.1
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隶属关系:
- Institute of Protein Research
- 期: 卷 81, 编号 10 (2016)
- 页面: 1198-1204
- 栏目: Accelerated Publication
- URL: https://journals.rcsi.science/0006-2979/article/view/151057
- DOI: https://doi.org/10.1134/S0006297916100175
- ID: 151057
如何引用文章
详细
The formation of ribosomal 48S initiation complexes at the start AUG codon of uncapped mRNA leader sequences was studied using the methodology of primer extension inhibition (toe-printing). The experiments were performed in the system composed of purified individual components required for translation initiation. The formation of ribosomal 48S initiation complexes at the initiation codon was tested depending on the presence of the initiation factors eIF4F, eIF4A, and eIF4B. Several mRNAs containing short leader sequences lacking the extended secondary structure were studied. It was found that 48S ribosomal complexes at mRNAs with such leaders were not formed in the absence of eIF4F. In contrast, the removal of either eIF4A or eIF4B from the experimental system was found to be dispensable for the formation of the 48S complex.
作者简介
P. Sakharov
Institute of Protein Research
Email: sultan@vega.protres.ru
俄罗斯联邦, Pushchino, Moscow Region, 142290
S. Agalarov
Institute of Protein Research
编辑信件的主要联系方式.
Email: sultan@vega.protres.ru
俄罗斯联邦, Pushchino, Moscow Region, 142290
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