Connection between proliferation rate and temozolomide sensitivity of primary glioblastoma cell culture and expression of YB-1 and LRP/MVP
- 作者: Moiseeva N.1, Susova O.1, Mitrofanov A.1, Panteleev D.2, Pavlova G.2, Pustogarov N.2, Stavrovskaya A.1, Rybalkina E.1
-
隶属关系:
- Blokhin Russian Cancer Research Center
- Institute of Gene Biology
- 期: 卷 81, 编号 6 (2016)
- 页面: 628-635
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150923
- DOI: https://doi.org/10.1134/S0006297916060109
- ID: 150923
如何引用文章
详细
Glioblastomas (GBL) are the most common and aggressive brain tumors. They are distinguished by high resistance to radiation and chemotherapy. To find novel approaches for GBL classification, we obtained 16 primary GBL cell cultures and tested them with real-time PCR for mRNA expression of several genes (YB-1, MGMT, MELK, MVP, MDR1, BCRP) involved in controlling cell proliferation and drug resistance. The primary GBL cultures differed in terms of proliferation rate, wherein a group of GBL cell cultures with low proliferation rate demonstrated higher resistance to temozolomide. We found that GBL primary cell cultures characterized by high proliferation rate and lower resistance to temozolomide expressed higher mRNA level of the YB-1 and MDR1 genes, whereas upregulated expression of MVP/LRP mRNA was a marker in the group of GBL with low proliferation rate and high resistance. A moderate correlation between expression of YB-1 and MELK as well as YB-1 and MDR1 was found. In the case of YB-1 and MGMT expression, no correlation was found. A significant negative correlation was revealed between mRNA expression of MVP/LRP and MELK, MDR1, and BCRP. No correlation in expression of YB-1 and MVP/LRP genes was observed. It seems that mRNA expression of YB-1 and MVP/LRP may serve as a marker for GBL cell cultures belonging to distinct groups, each of which is characterized by a unique pattern of gene activity.
作者简介
N. Moiseeva
Blokhin Russian Cancer Research Center
编辑信件的主要联系方式.
Email: N.I.Moiseeva@gmail.com
俄罗斯联邦, Moscow, 115487
O. Susova
Blokhin Russian Cancer Research Center
Email: Kate_Rybalkina@mail.ru
俄罗斯联邦, Moscow, 115487
A. Mitrofanov
Blokhin Russian Cancer Research Center
Email: Kate_Rybalkina@mail.ru
俄罗斯联邦, Moscow, 115487
D. Panteleev
Institute of Gene Biology
Email: Kate_Rybalkina@mail.ru
俄罗斯联邦, Moscow, 119334
G. Pavlova
Institute of Gene Biology
Email: Kate_Rybalkina@mail.ru
俄罗斯联邦, Moscow, 119334
N. Pustogarov
Institute of Gene Biology
Email: Kate_Rybalkina@mail.ru
俄罗斯联邦, Moscow, 119334
A. Stavrovskaya
Blokhin Russian Cancer Research Center
Email: Kate_Rybalkina@mail.ru
俄罗斯联邦, Moscow, 115487
E. Rybalkina
Blokhin Russian Cancer Research Center
编辑信件的主要联系方式.
Email: Kate_Rybalkina@mail.ru
俄罗斯联邦, Moscow, 115487
![](/img/style/loading.gif)