Application of created restriction site PCR-RFLP to identify POT1 gene polymorphism
- 作者: Wang T.1, Wang S.1,2, Duan X.1, Feng X.1, Wang P.1, Yao W.1, Wu Y.3, Feng F.3, Yu S.4, Wu Y.1, Wang W.1
-
隶属关系:
- College of Public Health, Department of Occupational Health and Occupational Disease
- Department of Occupational Health
- College of Public Health, Department of Hygiene Toxicology
- College of Public Health, Department of Sanitary Chemistry
- 期: 卷 81, 编号 6 (2016)
- 页面: 624-627
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150918
- DOI: https://doi.org/10.1134/S0006297916060092
- ID: 150918
如何引用文章
详细
Protection of telomeres protein 1 (POT1) plays pivotal roles in protection of chromosome ends and regulation of telomere length with other telomere binding proteins; its genetic polymorphisms are associated with many diseases. In this study, we explored a novel PCR-RFLP method for typing the single nucleotide polymorphism (SNP) rs1034794 of the human POT1 gene. A new restriction enzyme site was introduced into a POT1 gene amplification product by created restriction site PCR (CRS-PCR). One primer was designed based on changed sequence; after PCR amplification, a new restriction enzyme site for AluI was introduced into the PCR products. One hundred and seventy eight samples from Han Chinese individuals were tested to evaluate this new method. The 3′-end of the forward primer was next to the polymorphic site, and the third base from the 3′-end was the mismatched base A. The final PCR product contained the AGCT sequence (AluI recognition site) when the ancestral POT1 alleles were amplified. The data obtained with the new method perfectly matched those obtained with the sequencing method. Thus, CRS-PCR is a new low-cost and high-efficiency alternative for rs1034794 typing.
作者简介
Tuanwei Wang
College of Public Health, Department of Occupational Health and Occupational Disease
Email: ww375@126.com
中国, Zhengzhou, 450001
Sihua Wang
College of Public Health, Department of Occupational Health and Occupational Disease; Department of Occupational Health
Email: ww375@126.com
中国, Zhengzhou, 450001; Zhengzhou, 450052
Xiaoran Duan
College of Public Health, Department of Occupational Health and Occupational Disease
Email: ww375@126.com
中国, Zhengzhou, 450001
Xiaolei Feng
College of Public Health, Department of Occupational Health and Occupational Disease
Email: ww375@126.com
中国, Zhengzhou, 450001
Pengpeng Wang
College of Public Health, Department of Occupational Health and Occupational Disease
Email: ww375@126.com
中国, Zhengzhou, 450001
Wu Yao
College of Public Health, Department of Occupational Health and Occupational Disease
Email: ww375@126.com
中国, Zhengzhou, 450001
Yongjun Wu
College of Public Health, Department of Hygiene Toxicology
Email: ww375@126.com
中国, Zhengzhou, 450001
Feifei Feng
College of Public Health, Department of Hygiene Toxicology
Email: ww375@126.com
中国, Zhengzhou, 450001
Songcheng Yu
College of Public Health, Department of Sanitary Chemistry
Email: ww375@126.com
中国, Zhengzhou, 450001
Yiming Wu
College of Public Health, Department of Occupational Health and Occupational Disease
Email: ww375@126.com
中国, Zhengzhou, 450001
Wei Wang
College of Public Health, Department of Occupational Health and Occupational Disease
编辑信件的主要联系方式.
Email: ww375@126.com
中国, Zhengzhou, 450001
![](/img/style/loading.gif)