Na+-translocating rhodopsin from Dokdonia sp. PRO95 does not contain carotenoid antenna
- 作者: Bertsova Y.1, Arutyunyan A.1, Bogachev A.1
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隶属关系:
- Belozersky Institute of Physico-Chemical Biology
- 期: 卷 81, 编号 4 (2016)
- 页面: 414-419
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150845
- DOI: https://doi.org/10.1134/S000629791604012X
- ID: 150845
如何引用文章
详细
Carotenoid-binding properties of Na+-translocating rhodopsin (NaR) from Dokdonia sp. PRO95 were studied. Carotenoids were extracted from Dokdonia sp. PRO95 cells. It was found that zeaxanthin is the predominant carotenoid of this bacterium. Incubation of recombinant NaR purified from Escherichia coli cells with carotenoids from Dokdonia sp. PRO95 did not result in any changes in optical absorption or circular dichroism spectra, indicating the absence of binding of the carotenoids by NaR. The same results were obtained using salinixanthin as the carotenoid. These data along with genome analysis of Dokdonia sp. PRO95 and other flavobacteria indicate that NaR from Dokdonia sp. PRO95 and possibly the other flavobacterial Na+-translocating rhodopsins do not contain a carotenoid antenna.
作者简介
Y. Bertsova
Belozersky Institute of Physico-Chemical Biology
Email: bogachev@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
A. Arutyunyan
Belozersky Institute of Physico-Chemical Biology
Email: bogachev@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
A. Bogachev
Belozersky Institute of Physico-Chemical Biology
编辑信件的主要联系方式.
Email: bogachev@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991