Identification of a region of the polypeptide chain of Na,K-ATPase α-subunit interacting with 67-kDa melittin-like protein
- 作者: Kamanina Y.1,2, Klimanova E.1,2, Dergousova E.1,2, Petrushanko I.2, Lopina O.1
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隶属关系:
- Department of Biochemistry, School of Biology
- Engelhardt Institute of Molecular Biology
- 期: 卷 81, 编号 3 (2016)
- 页面: 249-254
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150802
- DOI: https://doi.org/10.1134/S000629791603007X
- ID: 150802
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详细
It was shown earlier that a 67-kDa protein purified from mouse kidney using polyclonal antibodies against melittin (a peptide from bee venom) interacted with Na,K-ATPase from rabbit kidney. In this study, a 43-kDa proteolytic fragment of Na,K-ATPase α-subunit interacting with the 67-kDa melittin-like protein was found. The α-subunit was hydrolyzed by trypsin in the presence of 0.5 mM ouabain (E2-conformation of Na,K-ATPase). A proteolytic fragment interacting with the 67-kDa melittin-like protein that was identified by mass-spectrometry is a region of the cytoplasmic domain of Na,K-ATPase α-subunit located between amino acid residues 591 and 775. The fragment includes a conservative DPPRA motif that occurs in many P-type ATPases. It was shown earlier that this motif of H,K-ATPase from gastric mucosa binds to melittin. We suggest that namely this motif of P-type ATPases is able to interact with proteins containing melittin-like modules.
作者简介
Yu. Kamanina
Department of Biochemistry, School of Biology; Engelhardt Institute of Molecular Biology
Email: od_lopina@mail.ru
俄罗斯联邦, Moscow, 119991; Moscow, 119991
E. Klimanova
Department of Biochemistry, School of Biology; Engelhardt Institute of Molecular Biology
Email: od_lopina@mail.ru
俄罗斯联邦, Moscow, 119991; Moscow, 119991
E. Dergousova
Department of Biochemistry, School of Biology; Engelhardt Institute of Molecular Biology
Email: od_lopina@mail.ru
俄罗斯联邦, Moscow, 119991; Moscow, 119991
I. Petrushanko
Engelhardt Institute of Molecular Biology
Email: od_lopina@mail.ru
俄罗斯联邦, Moscow, 119991
O. Lopina
Department of Biochemistry, School of Biology
编辑信件的主要联系方式.
Email: od_lopina@mail.ru
俄罗斯联邦, Moscow, 119991
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