Glutathione-dependent formaldehyde dehydrogenase homolog from Bacillus subtilis strain R5 is a propanol-preferring alcohol dehydrogenase
- Авторы: Ashraf R.1, Rashid N.1, Basheer S.1, Aziz I.1, Akhtar M.1
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Учреждения:
- School of Biological Sciences
- Выпуск: Том 82, № 1 (2017)
- Страницы: 13-23
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151212
- DOI: https://doi.org/10.1134/S0006297917010023
- ID: 151212
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Аннотация
Genome search of Bacillus subtilis revealed the presence of an open reading frame annotated as glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase. The open reading frame consists of 1137 nucleotides corresponding to a polypeptide of 378 amino acids. To examine whether the encoded protein is glutathione-dependent formaldehyde dehydrogenase or alcohol dehydrogenase, we cloned and characterized the gene product. Enzyme activity assays revealed that the enzyme exhibits a metal ion-dependent alcohol dehydrogenase activity but no glutathione-dependent formaldehyde dehydrogenase or aldehyde dismutase activity. Although the protein is of mesophilic origin, optimal temperature for the enzyme activity is 60°C. Thermostability analysis by circular dichroism spectroscopy revealed that the protein is stable up to 60°C. Presence or absence of metal ions in the reaction mixture did not affect the enzyme activity. However, metal ions were necessary at the time of protein production and folding. There was a marked difference in the enzyme activity and CD spectra of the proteins produced in the presence and absence of metal ions. The experimental results obtained in this study demonstrate that the enzyme is a bona-fide alcohol dehydrogenase and not a glutathionedependent formaldehyde dehydrogenase.
Об авторах
Raza Ashraf
School of Biological Sciences
Email: naeem.ff.sbs@pu.edu.pk
Пакистан, Lahore, 54590
Naeem Rashid
School of Biological Sciences
Автор, ответственный за переписку.
Email: naeem.ff.sbs@pu.edu.pk
Пакистан, Lahore, 54590
Saadia Basheer
School of Biological Sciences
Email: naeem.ff.sbs@pu.edu.pk
Пакистан, Lahore, 54590
Iram Aziz
School of Biological Sciences
Email: naeem.ff.sbs@pu.edu.pk
Пакистан, Lahore, 54590
Muhammad Akhtar
School of Biological Sciences
Email: naeem.ff.sbs@pu.edu.pk
Великобритания, Southampton, SO16 7PX