Modulation of δ-aminolevulinic acid dehydratase activity by the sorbitol-induced osmotic stress in maize leaf segments
- Autores: Jain M.1, Tiwary S.1, Gadre R.1
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Afiliações:
- School of Biochemistry
- Edição: Volume 83, Nº 1 (2018)
- Páginas: 32-36
- Seção: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151565
- DOI: https://doi.org/10.1134/S0006297918010042
- ID: 151565
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Resumo
Osmotic stress induced with 1 M sorbitol inhibited δ-aminolevulinic acid dehydratase (ALAD) and aminolevulinic acid (ALA) synthesizing activities in etiolated maize leaf segments during greening; the ALAD activity was inhibited to a greater extent than the ALA synthesis. When the leaves were exposed to light, the ALAD activity increased for the first 8 h, followed by a decrease observed at 16 and 24 h in both sorbitol-treated and untreated leaf tissues. The maximum inhibition of the enzyme activity was observed in the leaf segments incubated with sorbitol for 4 to 8 h. Glutamate increased the ALAD activity in the in vitro enzymatic preparations obtained from the sorbitol-treated leaf segments; sorbitol inhibited the ALAD activity in the preparations from both sorbitol-treated and untreated leaves. It was suggested that sorbitol-induced osmotic stress inhibits the enzyme activity by affecting the ALAD induction during greening and regulating the ALAD steady-state level of ALAD in leaf cells. The protective effect of glutamate on ALAD in the preparations from the sorbitol-treated leaves might be due to its stimulatory effect on the enzyme.
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Sobre autores
M. Jain
School of Biochemistry
Autor responsável pela correspondência
Email: mjjainmeeta@gmail.com
Índia, Khandwa Road, Indore (M.P.), 452001
S. Tiwary
School of Biochemistry
Email: mjjainmeeta@gmail.com
Índia, Khandwa Road, Indore (M.P.), 452001
R. Gadre
School of Biochemistry
Email: mjjainmeeta@gmail.com
Índia, Khandwa Road, Indore (M.P.), 452001