Microbiota induces expression of tumor necrosis factor in postnatal mouse skin
- Autores: Yuzhakova D.1,2, Shirmanova M.1, Bocharov A.3, Astrakhantseva I.2, Vasilenko E.2, Gorshkova E.2, Drutskaya M.3, Zagaynova E.1, Nedospasov S.2,3,4,5, Kruglov A.2,4,5
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Afiliações:
- Nizhny Novgorod State Medical Academy
- Lobachevsky State University of Nizhny Novgorod
- Engelhardt Institute of Molecular Biology
- Belozersky Institute of Physico-Chemical Biology
- Faculty of Biology
- Edição: Volume 81, Nº 11 (2016)
- Páginas: 1303-1308
- Seção: Molecular and Cellular Mechanisms of Inflammation (Special Issue) Guest Editors S. A. Nedospasov and D. V. Kuprash
- URL: https://journals.rcsi.science/0006-2979/article/view/151088
- DOI: https://doi.org/10.1134/S0006297916110080
- ID: 151088
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Resumo
Tumor necrosis factor (TNF) is a pleiotropic cytokine that regulates many important processes in the body. TNF production in a physiological state supports the structure of lymphoid organs and determines the development of lymphoid cells in hematopoiesis. However, chronic TNF overexpression leads to the development of various autoimmune disorders. Sites of TNF production in the naive state remain unclear due to the lack of in vivo models. In the present study, we used TNF-2A-Kat reporter mice to monitor the expression of TNF in different tissues. Comparative analysis of tissue fluorescence in TNF-2A-Kat reporter mice and wild type mice revealed constitutive expression of TNF in the skin of naive adult mice. In the skin of TNF-2A-Kat reporter mouse embryos, no statistically significant differences in the expression of TNF compared to wild type animals were observed. Furthermore, we established that local depletion of microflora with topical antibiotics leads to a reduction in the fluorescence signal. Thus, we assume that the skin microflora is responsible for the expression of TNF in the skin of mice.
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Sobre autores
D. Yuzhakova
Nizhny Novgorod State Medical Academy; Lobachevsky State University of Nizhny Novgorod
Email: andrey_krugloff@mail.ru
Rússia, Nizhny Novgorod, 603005; Nizhny Novgorod, 603950
M. Shirmanova
Nizhny Novgorod State Medical Academy
Email: andrey_krugloff@mail.ru
Rússia, Nizhny Novgorod, 603005
A. Bocharov
Engelhardt Institute of Molecular Biology
Email: andrey_krugloff@mail.ru
Rússia, Moscow, 119991
I. Astrakhantseva
Lobachevsky State University of Nizhny Novgorod
Email: andrey_krugloff@mail.ru
Rússia, Nizhny Novgorod, 603950
E. Vasilenko
Lobachevsky State University of Nizhny Novgorod
Email: andrey_krugloff@mail.ru
Rússia, Nizhny Novgorod, 603950
E. Gorshkova
Lobachevsky State University of Nizhny Novgorod
Email: andrey_krugloff@mail.ru
Rússia, Nizhny Novgorod, 603950
M. Drutskaya
Engelhardt Institute of Molecular Biology
Email: andrey_krugloff@mail.ru
Rússia, Moscow, 119991
E. Zagaynova
Nizhny Novgorod State Medical Academy
Email: andrey_krugloff@mail.ru
Rússia, Nizhny Novgorod, 603005
S. Nedospasov
Lobachevsky State University of Nizhny Novgorod; Engelhardt Institute of Molecular Biology; Belozersky Institute of Physico-Chemical Biology; Faculty of Biology
Email: andrey_krugloff@mail.ru
Rússia, Nizhny Novgorod, 603950; Moscow, 119991; Moscow, 119991; Moscow, 119991
A. Kruglov
Lobachevsky State University of Nizhny Novgorod; Belozersky Institute of Physico-Chemical Biology; Faculty of Biology
Autor responsável pela correspondência
Email: andrey_krugloff@mail.ru
Rússia, Nizhny Novgorod, 603950; Moscow, 119991; Moscow, 119991
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