Plasticity of Human THP–1 Cell Phagocytic Activity during Macrophagic Differentiation
- Авторлар: Kurynina A.1, Erokhina M.1,2, Makarevich O.1, Sysoeva V.1, Lepekha L.2, Kuznetsov S.3, Onishchenko G.1
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Мекемелер:
- Lomonosov Moscow State University
- Central Tuberculosis Research Institute
- Institute of Biological Sciences
- Шығарылым: Том 83, № 3 (2018)
- Беттер: 200-214
- Бөлім: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151608
- DOI: https://doi.org/10.1134/S0006297918030021
- ID: 151608
Дәйексөз келтіру
Аннотация
Studies of the role of macrophages in phagocytosis are of great theoretical and practical importance for understanding how these cells are involved in the organism’s defense response and in the development of various pathologies. Here we investigated phagocytic plasticity of THP–1 (acute monocytic human leukemia) cells at different stages (days 1, 3, and 7) of phorbol ester (PMA)–induced macrophage differentiation. Analysis of cytokine profiles showed that PMA at a concentration of 100 nM induced development of the proinflammatory macrophage population. The functional activity of macrophages was assessed on days 3 and 7 of differentiation using unlabeled latex beads and latex beads conjugated with ligands (gelatin, mannan, and IgG Fc fragment) that bind to the corresponding specific receptors. The general phagocytic activity increased significantly (1.5–2.0–fold) in the course of differentiation; phagocytosis occurred mostly through the Fc receptors, as shown previously for M1 macrophages. On day 7, the levels of phagocytosis of gelatin-and Fc–covered beads were high; however, the intensity of ingestion of mannan–conjugated beads via mannose receptors increased 2.5–3.0–fold as well, which indicated formation of cells with an alternative phenotype similar to that of M2 macrophages. Thus, the type and the plasticity of phagocytic activity at certain stages of macrophage differentiation can be associated with the formation of functionally mature morphological phenotype. This allows macrophages to exhibit their phagocytic potential in response to specific ligands. These data are of fundamental importance and can be used to develop therapeutic methods for correcting the M1/M2 macrophage ratio in an organism.
Авторлар туралы
A. Kurynina
Lomonosov Moscow State University
Email: masha.erokhina@gmail.com
Ресей, Moscow, 119991
M. Erokhina
Lomonosov Moscow State University; Central Tuberculosis Research Institute
Хат алмасуға жауапты Автор.
Email: masha.erokhina@gmail.com
Ресей, Moscow, 119991; Moscow, 107564
O. Makarevich
Lomonosov Moscow State University
Email: masha.erokhina@gmail.com
Ресей, Moscow, 119192
V. Sysoeva
Lomonosov Moscow State University
Email: masha.erokhina@gmail.com
Ресей, Moscow, 119192
L. Lepekha
Central Tuberculosis Research Institute
Email: masha.erokhina@gmail.com
Ресей, Moscow, 107564
S. Kuznetsov
Institute of Biological Sciences
Email: masha.erokhina@gmail.com
Германия, Rostock, 18051
G. Onishchenko
Lomonosov Moscow State University
Email: masha.erokhina@gmail.com
Ресей, Moscow, 119991
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