Sodium orthovanadate inhibits proliferation and triggers apoptosis in oral squamous cell carcinoma in vitro
- Авторлар: Khalil A.1,2, Jameson M.1
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Мекемелер:
- Department of Otolaryngology, Head and Neck Surgery
- National Liver Institute, Department of Biochemistry
- Шығарылым: Том 82, № 2 (2017)
- Беттер: 149-155
- Бөлім: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151246
- DOI: https://doi.org/10.1134/S0006297917020067
- ID: 151246
Дәйексөз келтіру
Аннотация
Sodium orthovanadate (SOV) is a general inhibitor of tyrosine phosphatases, a large family of enzymes that catalyze the removal of phosphate groups from tyrosine residues. SOV is commonly used in the laboratory to preserve the protein tyrosyl phosphorylation state of proteins under study. It has shown promising antineoplastic activity in some human cancer cell lines; this effect has not been fully investigated in head and neck squamous cell carcinoma. In this study, the effect of SOV on cell growth, proliferation, viability, and apoptosis was assessed in Cal27 cells, an oral squamous cell carcinoma (OSCC) cell line. SOV exhibited dose-dependent inhibition of cell growth and decrease in cell viability and colony formation. The IC50 values for treatment lasting 72 h and 7 days were 25 and 10 μM, respectively. The cytotoxic effect of the drug was associated with poly(ADP-ribose)polymerase cleavage detected by immunoblot. Flow cytometry of Cal27 cells stained with annexin V-FITC and propidium iodide showed a dose-dependent increase in apoptosis that reached approximately 40% at 25 μM SOV. These findings demonstrate that SOV has in vitro antiproliferative and proapoptotic effect on OSCC cells.
Негізгі сөздер
Авторлар туралы
A. Khalil
Department of Otolaryngology, Head and Neck Surgery; National Liver Institute, Department of Biochemistry
Хат алмасуға жауапты Автор.
Email: mark.jameson@virginia.edu
АҚШ, Charlottesville, Virginia, 1215; Menoufiya
M. Jameson
Department of Otolaryngology, Head and Neck Surgery
Email: mark.jameson@virginia.edu
АҚШ, Charlottesville, Virginia, 1215
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