Pseudogene PTENP1 functions as a competing endogenous RNA (ceRNA) to regulate PTEN expression by sponging miR-499-5p
- Authors: Wang L.1, Zhang N.2, Wang Z.1, Ai D.1, Cao Z.1, Pan H.3
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Affiliations:
- Second Medical School, Department of Rehabilitation Medicine
- Nanjing Drum Tower Hospital, Department of Cardiology
- Department of Rehabilitation Medicine
- Issue: Vol 81, No 7 (2016)
- Pages: 739-747
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150947
- DOI: https://doi.org/10.1134/S0006297916070105
- ID: 150947
Cite item
Abstract
Increasing evidence has shown that pseudogenes can widely regulate gene expression. However, little is known about the specific role of PTENP1 and miR-499-5p in insulin resistance. The relative transcription level of PTENP1 was examined in db/db mice and high fat diet (HFD)-fed mice by real-time PCR. To explore the effect of PTENP1 on insulin resistance, adenovirus overexpressing or inhibiting vectors were injected through the tail vein. Bioinformatics predictions and a luciferase reporter assay were used to explore the interaction between PTENP1 and miR-499-5p. The relative transcription level of PTENP1 was largely enhanced in db/db mice and HFD-fed mice. Furthermore, the overexpression of PTENP1 resulted in impaired Akt/GSK activation as well as glycogen synthesis, while PTENP1 inhibition led to the improved activation of Akt/GSK and enhanced glycogen contents. More importantly, PTENP1 could directly bind miR-499-5p, thereby becoming a sink for miR-499-5p. PTENP1 overexpression results in the impairment of the insulin-signaling pathway and may function as a competing endogenous RNA for miR-499-5p, thereby contributing to insulin resistance.
Keywords
About the authors
Lei Wang
Second Medical School, Department of Rehabilitation Medicine
Author for correspondence.
Email: Wanglei150306@163.com
China, Nanjing, 210023
Ning Zhang
Nanjing Drum Tower Hospital, Department of Cardiology
Email: Wanglei150306@163.com
China, Nanjing, 210093
Zun Wang
Second Medical School, Department of Rehabilitation Medicine
Email: Wanglei150306@163.com
China, Nanjing, 210023
Dong-mei Ai
Second Medical School, Department of Rehabilitation Medicine
Email: Wanglei150306@163.com
China, Nanjing, 210023
Zhen-yu Cao
Second Medical School, Department of Rehabilitation Medicine
Email: Wanglei150306@163.com
China, Nanjing, 210023
Hua-ping Pan
Department of Rehabilitation Medicine
Email: Wanglei150306@163.com
China, Nanjing, 211100