Comparison of Xylanases of Various Origin Obtained in the Expression System of Pichia pastoris: Gene Expression, Biochemical Characteristics, and Biotechnological Potential

Resumo

Endo-1,4-β-xylanases from Bacillus pumilus VKPM В-7975, Paenibacillus polymyxa VKPM В-3015, Thermomyces lanuginosus VKPM F-224, and Schizophyllum commune VKPM F-433, which are members of a family of 11 glycohydrolases, have been obtained in the expression system of Pichia pastoris. Technologically valuable characteristics of the recombinant hydrolases, such as the specific activity, pH and thermal stability, temperature and pH optima, resistance to digestive enzymes and xylanase protein inhibitors from cereals, and substrate specificity were studied. It was shown that the enzyme from Paenibacillus polymyxa has the highest biotechnological potential for the creation of xylanases based on the recombinant Pichia pastoris yeast strain for their use in fodder production.